Rac1 G-LISA Activation Assay Kit BK128 from Cytoskeleton, Inc.

Product Specs
ItemRac1 G-LISA Activation Assay Kit
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberBK128
Detection TargetRac1
QuantityKIT 96 assays
MethodRac G-LISA Assay Kits
Brand/TrademarkCytoskeleton, Inc
ApplicationsELISA

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) The HACE1 E3 ligase mediates RAC1‐dependent control of mTOR signaling complexesEMBO reportsDecember 6, 2023Busra Turgu, Amal El‐Naggar, Melanie Kogler, Luigi Tortola, Hai‐Feng Zhang, Mariam Hassan, Michael M Lizardo, Sonia HY Kung, Wan Lam, Josef M Penninger, et al.activation assay This assay was performed according to manufacturer's detailed manual (Cytoskeleton, #BK128). 25 μg total of protein was added wells pre‐coated with RAC‐GTP‐binding protein. Then, the plate was

(2) Key role for Rac in the early transcriptional response to extracellular matrix stiffness and stiffness-dependent repression of ATF3Journal of cell scienceOctober 1, 2023Dang I, Brazzo JA, Bae Y, Assoian RK.Rac or Rho activity was assessed in duplicate using the G-LISA small G-protein activation assay kit (BK128, BK124, Cytoskeleton) according to the manufacturer's instructions. Briefly, serum-starved MEFs were

(3) The Rac inhibitor HV-107 as a potential therapeutic for metastatic breast cancerMolecular MedicineJune 14, 2023Grace Velez Crespo, Jescelica Ortiz, Eliud Hernández O’Farrill, Cornelis P. Vlaar, Mikhail Inyushin, Yuriy Kucheryavykh, Lilia Kucheryavykh5.2(LI-COR Biotechnology, Lincoln, NE, USA). Analysis of Rac activity on tumors was assessed with Rac Glisa(#BK128, Cytoskeleton, Denver, CO, USA) colorimetric assay as per manufacturer instructions and absorbance read

(4) FXR1 regulates vascular smooth muscle cell cytoskeleton, VSMC contractility, and blood pressure by multiple mechanismsCell reportsApril 25, 2023St Paul A, Corbett C, Peluzzo A, Kelemen S, Okune R, Haines DS, Preston K, Eguchi S, Autieri MV.Activation Kit,"Cytoskeleton, Inc",Cat #BK127 Rac1 G-LISA Activation Kit,"Cytoskeleton, Inc",Cat #BK128 ,, Deposited data,Deposited data,Deposited data ,, RNA-Immunoprecipitation Seq,This paper,N/A Mass spectrometry

(5) Pals1 functions in redundancy with SMAP1 to inhibit Arf6 in order to prevent Rac1-dependent colorectal cancer cell migration and invasionCancer gene therapyMarch 1, 2023Harms J, Lüttgenau SM, Emming C, Guske J, Weber K, Wagner T, Schowe L, Nedvetsky P, Krahn MP.(Tecan Trading AG). G-LISA Cells were cultured in 10 cm dishes until confluency. The Rac1 G-LISA activity assay (#BK128, Cytoskeleton Inc.) was performed according to manufacturer’s instructions, using 0.5 mg/ml protein

(6) PKCε inhibition prevents ischemia‑induced dendritic spine impairment in cultured primary neuronsExperimental and Therapeutic MedicineFebruary 16, 2023Chenjie Ge, Xuefeng Wang, Yunhong Wang, Lilei Lei, Guohua Song, Mincai Qian, Shiliang Wangactivity was measured using different G-LISA activation assay kits (cat. nos. BK124 for active RhoA, BK128 for active Rac1 and BK127 for CDC42; Cytoskeleton, Inc.) following the manufacturer's instructions.

(7) Thiopurines impair the apical plasma membrane expression of CFTR in pancreatic ductal cells via RAC1 inhibitionCellular and Molecular Life SciencesJanuary 7, 2023Bálint Tél, Noémi Papp, Árpád Varga, Viktória Szabó, Marietta Görög, Petra Susánszki, Tim Crul, Aletta Kis, Ingrid H. Sendstad, Mária Bagyánszki, et al.hydrochloride,Sigma-Aldrich,M6500 Ehop-016,Sigma-Aldrich,SML0526 NSC668394,Sigma-Aldrich,"341,216" RAC1 G-LISA activation assay,Cytoskeleton,BK128 alpha-amylase assay,Diagnosticum,"47,462" Cerulein,Tocris Bioscience,6264 "Apoptosis-necrosis detection kit (blue, green, red

(8) Stress Elicits Contrasting Effects on Rac1-Cofilin Signaling in the Hippocampus and AmygdalaFrontiers in Molecular NeuroscienceMay 3, 2022Mihika Bose, Mohammad Sarfaraz Nawaz, Rakhi Pal, Sumantra ChattarjiAssay The G-LISA Rac1 Activation Assay Biochem kit (Cytoskeleton Biochem kit; Denver, USA; catalog no. BK128) and RhoA Activation Assay Biochem kit (Cytoskeleton Biochem kit; Denver, USA; catalog no. BK124) were

(9) Investigating the Anti-Inflammatory Effects of RCI001 for Treating Ocular Surface Diseases: Insight Into the Mechanism of ActionFrontiers in ImmunologyMarch 24, 2022Seunghoon Kim, Ye Won Jang, Young-ah Ku, Yungyeong Shin, Md Mahbubur Rahman, Myung-Hee Chung, Yong Ho Kim, Dong Hyun Kim(1~1000 nM) for 2 h. The Rac1 activity assay was performed according to the manufacturer’s protocol (BK128, Cytoskeleton Inc., USA). In Vitro ELISA Briefly, RAW 264.7 cells were plated at a density of 8×104/well

(10) The Collagen Receptor Discoidin Domain Receptor 1b Enhances Integrin β1-Mediated Cell Migration by Interacting With Talin and Promoting Rac1 ActivationFrontiers in Cell and Developmental BiologyMarch 3, 2022Corina M. Borza et al.pull down. G-Lisa Activation Assay Rac1-GTP levels were measured using the colorimetric G-LISA kit (Cytoskeleton #BK128) following the manufacturer’s instructions. Briefly, 24 h serum starved cells were trypsinized and either

(11) Tripartite split-GFP assay to identify selective intracellular nanobody that suppresses GTPase RHOA subfamily downstream signalingFrontiers in immunologyJanuary 1, 2022Keller L, Tardy C, Ligat L, Le Pennec S, Bery N, Koraïchi F, Chinestra P, David M, Gence R, Favre G, et al.perform competition experiments using the G-LISA® procedure, we used G-LISA® RHOA (BK124) and RAC1 (BK128) assays (Cytoskeleton) according to the manufacturer’s instructions to assess if purified hs2dAb were

(12) Acetyl-CoA Carboxylase Inhibitor CP640.186 Increases Tubulin Acetylation and Impairs Thrombin-Induced Platelet AggregationInternational Journal of Molecular SciencesDecember 4, 2021Marie Octave et al.rotenone were from Agilent (Mito Stress Test Kit, #103015-100) (Santa Clara, CA, USA). Rac1 G-LISA kit (#BK128) was from Cytoskeleton, Inc, (Denver, CO, USA). Anti-phospho-cofilin (#3313), anti-cofilin (#5175),

(13) Attenuation of apoptotic cell detection triggers thymic regeneration after damageCell reportsOctober 5, 2021Kinsella S, Evandy CA, Cooper K, Iovino L, deRoos PC, Hopwo KS, Granadier DW, Smith CW, Rafii S, Dudakov JA.assays Activated Rac1 was measured using the absorbance-based G-LISA Rac1 Activation Assay Biochem Kit (BK128, Cytoskeleton, USA). Briefly, exECs were co-cultured with thymocytes harvested from untreated mice,

(14) Non-canonical glutamine transamination sustains efferocytosis by coupling redox buffering to oxidative phosphorylationNature MetabolismOctober 1, 2021Johanna Merlin et al.manufacturer’s instruction. Rac1 and Cdc42 activity assay Rac1 G-LISA Activation Assay Kit (Cytoskeleton BK128) and Cdc42 G-LISA Activation Assay Kit (Cytoskeleton BK127) was used according to the manufacturer’s

(15) Patient-derived iPSC-cerebral organoid modeling of the 17q11.2 microdeletion syndrome establishes CRLF3 as a critical regulator of neurogenesisCell reportsJuly 6, 2021Wegscheid ML, Anastasaki C, Hartigan KA, Cobb OM, Papke JB, Traber JN, Morris SM, Gutmann DH.Rho Activator II (CN-03; Cytoskeleton; CN03) to induce Rho activation. RhoA (BK124, Cytoskeleton) and Rac1 (BK128, Cytoskeleton) activity assays were performed on liquid nitrogen snap frozen NSC and hCO specimens,

(16) CLIC1 and CLIC4 mediate endothelial S1P receptor signaling to facilitate Rac1 and RhoA activity and functionScience signalingApril 20, 2021Mao Y, Kleinjan ML, Jilishitz I, Swaminathan B, Obinata H, Komarova YA, Bayless KJ, Hla T, Kitajewski JK.responses. G-LISA Rac1, RhoA, and Ras activation assays Assays were performed using Rac1 (Cytoskeleton, BK128), RhoA (Cytoskeleton, BK124), and Ras (Cytoskeleton BK131) G-LISA Activation Assay Kits. HUVECs were

(17) A role of PIEZO1 in iron metabolism in mice and humansCellFebruary 18, 2021Ma S, Dubin AE, Zhang Y, Mousavi SAR, Wang Y, Coombs AM, Loud M, Andolfo I, Patapoutian A.ELISA Kit,Intrinsic LifeSciences,SKU:ERF-200 Rac1G-LISA Activation Assay Kit,Cytoskeleton Inc.,SKU: BK128 Mouse Inflammatory Cytokines Multi-Analyte ELISArray Kits,Qiagen,Catalog#336161 Human Hepcidin Quantikine

(18) Ror2-mediated non-canonical Wnt signaling regulates Cdc42 and cell proliferation during tooth root developmentDevelopment (Cambridge, England)January 21, 2021Ma Y, Jing J, Feng J, Yuan Y, Wen Q, Han X, He J, Chen S, Ho TV, Chai Y.in tissue lysates was detected using Cdc42 or Rac1 G-LISA assay, respectively (Cytoskeleton, BK127/BK128). The level of activation was measured by reading at OD490 nm. TUNEL assays Apoptotic cells in mouse

(19) NF1-RAC1 axis regulates migration of the melanocytic lineageTranslational OncologyDecember 1, 2020Lionel Larribère, Yohanes Cakrapradipta Wibowo, Nitin Patil, Mohammed Abba, Isabel Tundidor, Rubén Gerardo Aguiñón Olivares, Heike Allgayer, Jochen Utikaldetection. Absorbance was read at 490 nm. Data are background subtracted (Cytoskeleton inc. Cat. # BK128). RAC1 activator (Cytoskeleton inc. CN04) or RAC1 inhibitor (Tocris Bioscience NSC 23766) were included

(20) Serotonin stimulated parathyroid hormone related protein induction in the mammary epithelia by transglutaminase-dependent serotonylationPLOS ONEOctober 23, 2020Celeste M. Sheftel, Laura L. HernandezIntracellular Rac1 activity was determined using a Rac1 G-LISA Activation Assay Kit (Cytoskeleton, #BK128, Denver, CO) using 25μg total protein per the manufacturer’s instructions. All assays had an intra-assay

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Rac1 G-LISA Activation Assay Kit from Cytoskeleton, Inc.

Cytoskeleton, Inc.

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Rac1 G-LISA Activation Assay Kit from Cytoskeleton, Inc.

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